Soybean variety AP98041-2-333

ABSTRACT

The present invention is in the field of soybean variety AP98041-2-333 breeding and development. The present invention particularly relates to the soybean variety AP98041-2-333 and its progeny, and methods of making AP98041-2-333.

THE FIELD OF THE INVENTION

[0001] The present invention is in the field of soybean varietyAP98041-2-333 breeding and development. The present inventionparticularly relates to the soybean variety AP98041-2-333 and itsprogeny, and methods of making.

BACKGROUND OF THE INVENTION

[0002] The breeding and development of crops has been ongoing across thelast 1000 years. The pace of this development in soybeans, as an animalfoodstuff and as an oil seed has dramatically increased in the last onehundred years. Planned programs of plant breeding have increased thegrowth, yield and environmental hardiness of the soybean germplasm. Dueto the sexual reproduction traits of the soybean the plant is basicallyself-pollinating. A self pollinating plant permits pollen from oneflower to be transferred to the same or another flower of the sameplant. Cross-pollination occurs when the flower is pollinated withpollen from a different plant. This is a rare occurrence in nature.

[0003] Thus the growth and development of new soybean germplasm requiresintervention by the breeder in the pollination of the soybean. Thebreeders' selections of methods of intervening in the pollination dependon the type of trait that is being selected. Soybeans are developed fora number of different types of traits morphological (form andstructure), phenotypical, for growth habit, daylength temperaturerequirements to initiate floral or reproductive development yield. Thegenetic complexity of the trait drives the breeding method. Backcrossbreeding is employed when the cultivar that is being bred has a fairlyfull profile of desirable traits, but lack one or two traits that arehighly inheritable. Backcrossing is often employed to move diseaseresistance, insect resistance and transgenes (hereinafter DNA which areintroduced into the original ancestor germplasm by a transformationmethod) into other varieties.

[0004] When the variety is being employed to develop a new variety or animproved variety the selection methods include pedigrees, recurrent,modified and mass selection and backcrossing. Each of these selectiontechniques is employed with the efficiency of the breeding procedure asthe driver. The breeding procedure requires a continuous evaluation ofthe success of the breeding program. The success is measured by yieldincrease, commercial appeal and environmental adaptability of thedeveloped germplasm.

[0005] New varieties must be tested thoroughly to compare thedevelopment with the commercially available soybeans. This testingusually requires at least two years and up to six years of comparisonswith other commercial soybeans. Varieties that lack the entire desirablepackage of traits can be used as parents in new populations for furtherselection. The breeding and associated testing process is 8 to 12 years'progression toward a new variety. Thousands of lines are produced andlimited lines are selected in each step of the process. Thus thebreeding system is like a funnel with numerous lines and selections inthe first few years and fewer and fewer lines in the middle years untilone line is selected for the final development testing.

[0006] The selected line or variety will be evaluated for it's thegrowth and development and yield. These traits of a soybean are a resultof the varieties genetic potential interacting with its environment. Allvarieties have a maximum yield potential that is predetermined by itsgenetics. This hypothetical potential for yield is only obtained whenthe environmental conditions are perfect. Since prefect growthconditions do not exist field experimentation is necessary to providethe environmental influence and to measure its effect on the developmentand yield of the soybean. The breeder attempts to select for goodsoybean yield potential under a number of different environmentalconditions.

[0007] Selecting for good soybean yield potential under a number ofdifferent environmental conditions is a process that requires planning,analysis of data in a number of seasons. Identification of the varietiesthat carry a superior combination of traits that provides thisconsistent yield potential is a complex science. Other plant traits,unusual weather patterns, diseases, and insect damage often mask thegenotypic traits. One widely employed method of identifying a superiorplant is to observe its performance relative to commercial andexperimental plants in replicated studies. These types of studies givemore certainty to the genetic potential and value of the plant.

[0008] The goal of the soybean plant breeder is to produce unique andnew soybeans and hybrids of the soybeans. To accomplish this the plantbreeder painstakingly crosses two or more varieties or germplasm. Thenthe results of this cross is repeatedly selfed or backcrossed to producenew genetic patterns. Additionally, the breeder can introduce mutationsinto the genetic material. These can alter herbicide resistance, fattyacid compositions, and amino acid compositions of the seeds and thelike. Fortunately, through transformation in combination with breedingthe plant breeder can alter or introduce some limited alleles into thebreeding material. This capability is widening the potential uses andmarkets for the various products and by products of oil seed plants suchas soybean. One of the products of soybeans is the oil of the seed.Soybean oil is employed in a number of retail products. Soybean meal isalso used in food and animal feedstuffs. The traits a breeder selectsfor can be driven by the ultimate goals of the end user of the product.Thus if the goals of the end user is to produce an oil with a high levelof oleic acid and a lower level of linoleic acid then the breeder maydrive the genetics toward levels of fatty acids and accept some lesseryield potentials or other less desirable agronomic traits.

[0009] Regardless of the market characteristics of the plant mostbreeding proceeds along a similar path on a yearly basis. The breederannually selects the germplasm to advance on into further development.This germplasm is grown in different locations at different altitudes,in different climates and subjected to different soil conditions basedon the datum collected individual plants are selected during the end ofthe growing season. Due to the number of genes within each chromosomemillions of genetic combinations exist in the breeders experimentalmaterial. This genetic diversity is so vast that a breeder cannotproduce the same two cultivars twice using the exact same material. Thusthe developing a single variety of useful commercial soybean germplasmis highly unpredictable, and requires intensive research.

[0010] The development of new soybeans comes through breeding techniquessuch as recurrent selection, mass selections, backcrossing, single seeddescent and multiple seed procedure that is used to save labor costs.Other breeding methods are taught in several soybean textbooks.

[0011] The development of soybean cultivars most often requires thedevelopment of hybrid crosses (the exception being initial developmentof mutants directly through the use of the mutating agent ortransformants directly through transformation methods) and the selectionof progeny therefrom. Hybrids can be achieved by manual manipulation ofthe sexual organs of the soybean or by the use of male sterilitysystems. The breeder attempts to identify true hybrids by a readilyidentifiable trait. These hybrids are then selected and repeatedlyselfed and selected to form new homozygous lines from the heterozygoushybrids.

[0012] Outcrossing to a number of different parents creates breedingpopulations of fairly heterozygous populations. These populations areproduced and used in pedigree breeding and recurrent selection. Pedigreebreeding is commonly used with two parents which possess favorable,complementary traits. The parents are crossed to form a F1 hybrid. Theprogeny of the F1 hybrid is selected from this the best individuals F2are selected; this is repeated in the F3 and F4 generations. Theinbreeding is carried forward and at F5-F7 the best lines are selectedand tested in the development stage for potential usefulness.

[0013] Mass and recurrent selection can be used to improve populations.Several parents are intercrossed and plants are selected based onselected characteristics like superiority or excellent progeny.

[0014] In backcross breeding a genetic allele or loci is transferredinto a desirable homozygous recurrent parent. The trait is in the donorparent and is tracked into the recurrent parent. The resultant plant islike the recurrent parent with the new desired allele or loci.

[0015] The single-seed descent method involves use of a segregatingplant population for harvest of one seed per plant. Each seed sample isplanted and the next generation is formed. When the F2 lines areadvanced to F6 each plant will be derived from a different F2. Thepopulation will decline due to failure of some seeds, so not all F2plants will be represented in the progeny.

[0016] Soybean Glycine max (L) is an important oil seed crop and avaluable field crop.

SUMMARY OF THE INVENTION

[0017] One embodiment of the invention relates to seed of a soybeancultivar designated AP98041-2-333. And the plant, or parts includingovule, a tissue culture of regenerable cells, cells or protoplasts beingfrom a tissue selected from the group consisting of leaves, pollen,embryos, meristematic cells, roots, root tips, anthers, flowers, seeds,stems and pods and pollen thereof, produced by growing the seed.

[0018] The invention in one aspect covers a soybean plant, or partsthereof, having all of the physiological and morphologicalcharacteristics of the soybean plant.

[0019] Another aspect of this invention is the soybean plant seed orderived progeny which contains a transgene (which include DNA whether ornot it is a full or partial sequence) affecting herbicide resistance,insect resistance, resistance to disease, nematodes, male sterility, andaltered oil or amino acids or other nutritional quality.

[0020] The present invention further covers a method for producing asoybean seed with the steps of crossing at least two parent soybeanplants and harvesting the hybrid soybean seed, wherein at least oneparent soybean plant is the present invention. In another aspect of theinvention covers the hybrid soybean seed and the progeny soybean plantand resultant seed, or parts thereof from the hybrid seed or plant orits progeny.

[0021] In an additional aspect the invention covers a method forproducing a soybean progeny from the invention by crossing soybean lineAP98041-2-333 with a second soybean plant to yield progeny soybean seedand then growing progeny soybean seed to develop a derived soybean line.

[0022] Yet another aspect of the invention covers a method for abreeding program using plant breeding techniques which employ thesoybean plant AP98041-2-333 as plant breeding material and performingbreeding by selection techniques, backcrossing, pedigree breeding,marker enhanced selection, mutation and transformation.

DETAILED DESCRIPTION Soybean Data Definitions

[0023] Following is a series of definitions of traits that are collectedas part of the research yield trial program. TRAIT DESCRIPTION TIMINGSCALE Core Traits - HILA COLOR (HC) Phenotypic observation Packaging G =Gray Br = Brown Ib = Imperfect Black Bl = Black Y = Yellow FLOWER COLORPhenotypic observation R1 W = White (FC) (Beginning P = Purple Bloom) X= Mix POD COLOR (POD) Phenotypic observation R8 T = Tan (Full Maturity)B = Brown X = Mixed PUBESCENCE Phenotypic observation R8 G = Gray COLOR(PUB) (Full Maturity) T = Tawny Lt = Tawny X = Mixed Core Traits - Takenon all Yield Trial Sets Included in harvest GWT Grain weight/plotHarvest Pounds H2O Grain moisture/plot Harvest % moisture MATURITY (MAT)# of days after Aug. 31 R8 Taken in days after Taken on all reps at when95% of the main (Full Maturity) Aug. 31 6 locations, stem pods in theplot have reached their mature color PLANT HEIGHT (HT) The averagemeasured Harvest Taken in inches Taken on all reps at plot height. 4locations where most growth occurs. STEM LODGING (LODGE) Taken whereverdifferential lodging occurs Rating based on the angle of the majority ofplants in the plot relative to the ground at 0° Harvest 1 = All erect 2= 67°3 = 45°4 = 22°5 = 0°

BRANCHING Rating of the number Harvest 1 = stick - no branch (BRANCH) ofbranches and their 2 = 1-2 branches relative importance to 3 = 2-3branches-average yield. Taken at 4 = >3-4 branches growth expressive 5= >5 branches - profuse locations SHATTER (SHAT) Rating of pre-harvestHarvest 1 = <1% of plot shattered Taken on tests where loses based on 2= 1-10% shattered shattering occurs percentage of plants 3 = 10-20%shattered with open pods 4 = 20-30% shattered 5 = >30% shatteredOptional Traits EMERGENCE (EMG) Rating of the uniform When 50% orSeedling Stages: establishment of more of the plants 1 = V1 (Uniform),seedling have reached V1 Stand > 85% (First leaf node) 2 = VC&V1 (lessuniform), VE(emergence) Stand > 85% VC(cotyledon 3 = VE&VC&V1,(variable) stage) Stand > 75% 4 = Gaps > 1′, Stand < 75% 5 = Gaps > 2′,Stand <= 50% GREEN LODGING (GLODGE) Rating based on the angle of themajority of plants in the plot R5 to R6 (Beginning seed to Full seed) 1= All erect 2 = 67°3 = 45°4 = 22°5 = 0°

Disease/Stress Traits PHYTOPHTHORA The observed reaction Field 1 to 5 (1= best) ROOT ROT of the variety in the Nursery or YT (PFT) presence ofthe organism (Yield Trial) plots BROWN STEM ROT The observed reactionField 1 to 5 (1 = best) (BSR) of the variety in the Nursery or presenceof the organism YT plots SCLEROTINIA WHITE The observed reaction Field 1to 5 (1 = best) MOLD of the variety in the Nursery or (SWM) presence ofthe organism YT plots SUDDEN DEATH The observed reaction Field 1 to 5 (1= best) SYNDROME of the variety in the Nursery or (SDS) presence of theorganism YT plots STEM CANKER The observed reaction Field 1 to 5 (1 =best) (STEMC) of the variety in the Nursery or presence of the organismYT plots CHARCOAL ROT The observed reaction Field 1 to 5 (1 = best)(CHROT) of the variety in the Nursery or presence of the organism YTplots FROG EYE (FRGEYE) The observed reaction Field 1 to 5 (1 = best) ofthe variety in the Nursery or presence of the organism YT plots IRONDEFICIENCY A composite rating of Hill Plots in 1 to 5 (1 = best)CHLOROSIS Yellow Flash, Green- soils of 7.9 pH or (IDC) up, and Stuntingin higher HpH(high pH) soil

[0024] Definitions of Staging of Development

[0025] The plant development staging system employed divides stages asvegetative (V) and reproductive (R). This system accurately identifiesthe stages of a soybean plant. However, all plants in a given field willnot be in the stage at the same time. Each specific V or R stage isdefined as when 50% or more of the plants in the field are in or beyondthat stage.

[0026] The first two stages of V are designated a VE (emergence) and VC(cotyledon stage). Subdivisions of the V stages are then designatednumerically as V1, V2, V3 through V (n) The last V stage is designatedas V (n), where (n) represents the number for the last node stage of thespecific variety. The (n) will vary with variety and environment. Theeight subdivisions of the reproductive stages (R) states are alsodesignated numerically. R1=beginning bloom; R2=full bloom; R3=beginningpod; R4=full pod; R5 =beginning seed; R6=Full Seed; R7=beginningmaturity; R8=Full maturity.

[0027] Brown Stem Rot (BSR)

[0028] This disease is caused by the fungus Phialophora gregata. Thedisease is a late-season, cool-temperature, soilborne which inappropriate favorable weather can cause up to 30 percent yield losses insoybean fields.

[0029] Sudden Death Syndrome (SDS)

[0030] This disease is caused by slow-growing strains of Fursariumsolani that produce bluish pigments in culture. The disease is mid- tolate season soil borne and occurs in soybean fields with high yieldpotential. Yield losses may be total I severely affected fields.

[0031] Soybean Cyst Nematode

[0032] The Soybean Cyst Nematode (SCN) Heterodera glycines, is a smallplant-parasitic roundworm that attacks the roots of soybeans.

[0033] Maturity Date.

[0034] Plants are considered mature when 95% of the pods have reachedtheir mature color. The number of days are either calculated fromSeptember 1 or from the planting date.

[0035] Relative Maturity Group (RM).

[0036] Industry Standard for varieties groups, based day length orlatitude. Long day length (northern areas in the Northern Hemisphere)are classified as (Groups 000,00,0,) and extend to very short daylengths variety groups (southern areas in Northern Hemisphere)classified as (Groups VII,VIII, IX).

[0037] Seed Yield (Bushels/Acre).

[0038] The yield in bushels/acre is the actual yield of the grain atharvest.

[0039] Shattering.

[0040] The rate of pod dehiscence prior to harvest. Pod dehiscenceinvolves beans dropping out of the pods.

[0041] Plant.

[0042] Means the plant cells, plant protoplasts, plant cells of tissueculture from which soybean plants can be regenerated, plant calli, plantclumps, and plant cells that are intact in plants or parts of plants,such as pollen, nodes, roots, flowers, seeds, pods, leaves, stems, andthe like.

[0043] The present invention is AP98041-2-333. This soybean is developedfor its use of the beans. AP98041-2-333 is a 2.6 relative maturity. Itbest adapted to regions in the U.S. (though it can be used in othercountries adapted for soybeans) of central Iowa, northern Illinois,northern Indiana, southern Michigan, northern Ohio, northern Nebraska,and extreme southern South Dakota. The traits of the invention arelisted below. TRAIT RM 2.6 HR-herbicide resistance RR = Round Up Readyresistance transgene Flower Color purple Pubescene Color T Pod ColorMixed Hila Color Black Seed/Lb 2700-3000 Lust Dull Perox PositivePhytophthora Root Rot 3.1 Rkn — Brown Stem Rot — Iron DeficiencyChlorosis 2.5 Sclerotinia White Mold — Sudden Death Syndrome — StemCanker — Charcoal Rot — Frog Eye — SCN R3-MR14 (Resistant race 3;moderately re- sistant race 13) PGR 1k

[0044] The instant invention provides methods and composition relatingto plants, seeds and derivatives of the soybean cultivar AP98041-2-333.Soybean cultivar AP98041-2-333 has superior characteristics. TheAP98041-2-333 line has been selfed sufficient number of generations toprovide a stable and uniform plant variety.

[0045] Cultivar AP98041-2-333 shows no variants other than expected dueto environment or that normally would occur for almost anycharacteristic during the course of repeated sexual reproduction. Someof the criteria used to select in various generations include: seedyield, lodging resistance, emergence, appearance, disease tolerance,maturity, plant height, maturity and shattering data.

[0046] The inventor believes that AP98041-2-333 is similar to thecomparison varieties. However, as shown in the tables, AP98041-2-333differs from these cultivars.

[0047] Direct comparisons were made between AP98041-2-333 and thesecompeting commercial varieties. Traits measured included yield,maturity, moisture, lodging, plant height, field emergence, protein andoil. The results of the comparison are presented in below. The number oftests in which the varieties were compared is shown. The deviation ordifference of the results, T-value and the traits which showed asignificant difference and the level of that significance are in thefirst table and the second Table indicates the advantage positive numberor disadvantage negative of the present invention when compared to thecommercial lines. The present invention AP98041-2-333 can carry theglyphosate resistance gene. The present invention AP98041-2-333 isemployed in a trialling for a number of characteristics. These testsallow the usefulness of the invention to be shown in light of theenvironmental genetic interactions. Ent Yld Moist Appearance Branch BSREmerge Lg Mat Pltht PRR Shatter IDC AP98041-2- 53.2 11.1 2.2 2 . . 1.516.5 31.3 . . . 333 AG2201 50.8 10.9 2.8 2 . . 2 11.9 33.5 . . . # REPS44 45 20 2 0 0 26 14   28 0 0 0 Diff 2.4 0.2 −0.7 0 . . −0.5  4.6 −2.2 .. . Std 8.8 0.7 1.2 0 . . 1.5  2.6 4.7 . . . T-val 1.77 1.73 −2.46 . . .−1.86  6.52 −2.48 . . . Prob 0.084* 0.091* 0.024** . . . 0.075*  0***0.02** . . .

[0048] The present invention is providing yield that is statisticalsignificant over the commercial line with a slightly higher moisture.

[0049] Although the present invention has a slightly later maturity indays after September 1 then the commercial line it has significantlyless lodging than does the commercial line in accordance to this test inthese environmental conditions.

[0050] This AP98041-2-333 invention was compared with D221 RR/N andD224RR/N for certain grain quality traits. The present invention showsthe same moisture as D221 RR/N and slightly higher moisture thanD224RR/N. The protein level of the present invention provide anadvantage over D221RR/N and the oil of AP98041-2-333 shows an advantageover the oil levels produced in the beans of D224RR/N. YEAR VARIETY #MOIST ADV PROTEIN ADV OIL ADV SEEDS/LB ADV 00 AP98041-2-333 2 6.7 041.75 1.59 21.28 −0.17 2874 −361.5 D221RR/N 2 6.7 40.16 21.45 3235.5 00AP98041-2-333 3 7.1 0.2 41.91 −0.5 21.29 0.27 2886 −322 D294RR/N 3 6.842.37 21.03 3208

[0051] This invention also is directed to methods for producing asoybean plant by crossing a first parent corn plant with a second parentplant wherein the first or second parent plant is present invention or aderived progeny therefrom. Further, both first and second parent plantscan come from the soybean line AP98041-2-333. A variety of breedingmethods can be selected depending on the mode of reproduction, thetrait, the condition of the germplasm. Thus, any such methods using theAP98041-2-333 are part of this invention: selfing, backcrosses,recurrent selection, mass selection and the like.

[0052] Transformation methods are means for integrating new geneticcoding sequences (transgenes) into the plant's genome by theincorporation of these sequences into a plant through man's assistance.Many dicots including soybeans can easily be transformed withAgrobacterium. The most common method of transformation after the use ofagrobacterium is referred to as gunning or microprojectile bombardment.This process has small gold-coated particles coated with DNA (includingthe transgene) shot into the transformable material. Techniques forgunning DNA into cells, tissue, explants, meristems, callus, embryos,and the like are well known in the prior art. The DNA used fortransformation of these plants clearly may be circular, linear, anddouble or single stranded. Usually, the DNA is in the form of a plasmid.The plasmid usually contains regulatory and/or targeting sequences whichassists the expression of the gene in the plant. The methods of formingplasmids for transformation are known in the art. Plasmid components caninclude such items as: leader sequences, transit polypeptides,promoters, terminators, genes, introns, marker genes, etc. Thestructures of the gene orientations can be sense, antisense, partialantisense, or partial sense: multiple gene copies can be used.

[0053] After the transformation of the plant material is complete, thenext step is identifying the cells or material, which has beentransformed. In some cases, a screenable marker is employed such as thebeta-glucuronidase gene of the uidA locus of E. coli. Then, thetransformed cells expressing the colored protein are selected for eitherregeneration or further use. In many cases, a selectable markeridentifies the transformed material. The putatively transformed materialis exposed to a toxic agent at varying concentrations. The cells nottransformed with the selectable marker, which provides resistance tothis toxic agent, die. Cells or tissues containing the resistantselectable marker generally proliferate. It has been noted that althoughselectable markers protect the cells from some of the toxic affects ofthe herbicide or antibiotic, the cells may still be slightly effected bythe toxic agent by having slower growth rates. If the transformedmaterial was cell lines then these lines are regenerated into plants.The cells' lines are treated to induce tissue differentiation. Methodsof regeneration of cellular are well known in the art. The plants fromthe transformation process or the plants resulting from a cross using atransformed line or the progeny of such plants are transgenic plantsthat carry the transgene.

[0054] Deposit Information

[0055] A deposit of the Advanta USA Inc. Seed soybean cultivarAP98041-2-333 disclosed above and recited in the appended claims will bemade with the American Type Culture Collection (ATCC), 10801 UniversityBoulevard, Manassas, Va.

[0056] 20110. The date of deposit was ______. The deposit of 2,500 seedsmaintained by Advanta USA Inc. since prior to the filing date of thisapplication. All restrictions upon the deposit have been removed, andthe deposit is intended to meet all of the requirements of 37 C.F.R..sctn.1.801-1.809. The ATCC accession number is ______. The deposit willbe maintained in the depository for a period of 30 years, or 5 yearsafter the last request, or for the effective life of the patent,whichever is longer, and will be replaced as necessary during thatperiod.

[0057] Accordingly, the present invention has been described with somedegree of particularity directed to the preferred embodiment of thepresent invention. It should be appreciated, though, that the presentinvention is defined by the following claims construed in light of theprior art so that modifications or changes may be made to the preferredembodiment of the present invention without departing from the inventiveconcepts contained herein.

What is claimed is:
 1. A soybean seed designated AP98041-2-333, a sampleof said seed deposited under ATCC Accession No. ______.
 2. A plant, orparts thereof, produced by growing the seed of claim
 1. 3. Pollen of theplant of claim
 2. 4. Oil of the seed of claim
 1. 5. A soybean plant, orparts thereof, having all of the physiological and morphologicalcharacteristics of the soybean plant of claim
 2. 6. A tissue culture ofregenerable cells of a soybean plant of cultivar AP98041-2-333, whereinthe tissue regenerates plants capable of expressing all of themorphological and physiological characteristics of the cultivarAP98041-2-333.
 7. A tissue culture according to claim 6, the cells orprotoplasts being from a tissue selected from the group consisting ofleaves, pollen, embryos, meristematic cells, roots, root tips, anthers,stomato cells, flowers, seeds, stems and pods.
 8. A soybean plantregenerated from the tissue culture of claim 6, capable of expressingall of the morphological and physiological characteristics of soybeancultivar AP98041-2-333.
 9. A method for producing a soybean seedcomprising crossing at least two soybean plants and harvesting theresultant soybean seed, wherein said at least one soybean plant is thesoybean plant of claim
 2. 10. A soybean plant, or parts thereof,produced by growing said soybean seed of claim
 9. 11. Soybean seedcomprising said soybean plant of claim 10 as at least one of itsancestors.
 12. A method for producing a hybrid soybean seed comprisingcrossing a soybean plant according to claim 2 with a second soybeanplant.
 13. Soybean seed comprising as one of its ancestors said hybridsoybean plant of claim
 12. 14. A hybrid soybean plant, or parts thereof,produced by growing said hybrid soybean seed of claim
 13. 15. A methodfor producing a AP98041-2-333-derived soybean plant, comprising: a)crossing soybean line AP98041-2-333 with a second soybean plant to yieldprogeny soybean seed; b) growing said progeny soybean seed to yield saidAP98041-2-333-derived soybean plant.
 16. A method according to claim 15wherein step c is harvesting the progeny soybean seed from the derivedsoybean plant and selecting progeny seed therefrom capable of forminganother AP98041-2-333-derived soybean plant., and step d is repeatingStep (c) at least once.
 17. A method for developing a soybean plant in asoybean plant breeding program using plant breeding techniques whichinclude employing a soybean plant as a source of plant breeding materialcomprising: using the soybean plant of claim 2 and wherein said plantbreeding techniques are selected from the group consisting of: recurrentselection, backcrossing, pedigree breeding, genetic marker enhancedselection, and transformation.
 18. A method of claim 9 with the step ofselecting at least one of the two soybean plants to have at least onetransgene.
 19. The method of claim 18 wherein the transgene is selectedfrom the group consisting of herbicide resistance, insect resistance,resistance to disease, male sterility, and altered oil, amino acid,nutritional quality, IMI resistant gene, glyphosate resistant gene, theoxalate oxidase encoding gene, the oxalate decarboxylate encoding gene.